한국자원식물학회:학술대회논문집 (Proceedings of the Plant Resources Society of Korea Conference)

한국자원식물학회 (The Plant Resources Society of Korea)
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허혈-재관류 유도 신경세포사멸에 대한 초석잠 추출물의 신경보호 효과 연구

Neuroprotective Effects of Stachys sieboldii Miq. Extract Against Ischemia/reperfusion-induced Apoptosis in SH-SY5Y Neuroblastoma Cells

  • Young-Kyung Lee (Nakdonggang National Institute of Biological Resources) ;
  • Chul Hwan Kim (Nakdonggang National Institute of Biological Resources) ;
  • Su Young Shin (Nakdonggang National Institute of Biological Resources) ;
  • Buyng Su Hwang (Nakdonggang National Institute of Biological Resources) ;
  • Min-Jeong Seo (Nakdonggang National Institute of Biological Resources) ;
  • Hye Jin Hwang (Nakdonggang National Institute of Biological Resources) ;
  • Kyung-Min Choi (Nakdonggang National Institute of Biological Resources) ;
  • Jin-Woo Jeong (Nakdonggang National Institute of Biological Resources)
  • 발행 : 2020.08.14
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초록

Stachys sieboldii Miq. (chinese artichoke), which has been extensively used in oriental traditional medicine to treat of ischemic stroke; however, the role of Stachys sieboldii Miq. (SSM) in cerebral ischemia/reperfusion (I/R) injury is not yet fully understood. In the current study, the neuroblastoma cell line (SH-SY5Y) were subjected to oxygen-glucose deprivation/reperfusion (OGD/R) to simulate I/R injury in vitro model. The results showed that SSM improved OGD/R-induced inhibitory effect on cell viability of SH-SY5Y Cells. SSM displayed anti-oxidative activity as proved by the decreased levels of reactive oxygen species (ROS) and malondialdehyde (MDA), and increased activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in OGD/R-induced SH-SY5Y Cells. In addition, cell apoptosis was markedly decreased after SSM treatment in OGD/R-induced SH-SY5Y Cells. The up-regulation of Bcl-2 and down-regulation of Bax, thus reducing the Bax/Bcl-2 ratio that in turn protected the activation of caspase-9 and -3, and inhibition of poly (ADP-ribose) polymerase cleavage, which was associated with the blocking of cytochrome c release to the cytoplasm. Collectively, SSM protected human neuroblastoma SH-SY5Y cells from OGD/R-induced injury via preventing mitochondrial-dependent pathway through scavenging excessive ROS, suggesting that SSM might be a potential agent for the ischemic stroke therapy.

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과제정보

본 논문은 환경부의 재원으로 국립낙동강생물자원관(NNIBR202002106)의 지원을 받아 수행된 연구이며, 이에 감사드립니다.