Identification of genes related to ER stress in bZIP28 gene transgenic potato plant

  • Kim, Dool Yi (Crop Foundation Research Division, National Institute of Crop Science, RDA) ;
  • Kim, Kyung Hwa (Crop Foundation Research Division, National Institute of Crop Science, RDA) ;
  • Choi, Man Soo (Crop Foundation Research Division, National Institute of Crop Science, RDA) ;
  • Ok, Hyun Choog (Crop Foundation Research Division, National Institute of Crop Science, RDA) ;
  • Kim, Jae Hyun (Crop Foundation Research Division, National Institute of Crop Science, RDA)
  • Published : 2017.06.04

Abstract

Potato (Solanum tubersosum L.) is susceptible to various environmental stresses such as frost, high temperature, and drought. Enhancement of potato drought tolerance can reduce yield loss under drought that has negative effect on potato tuber growth. Genetic engineering can be utilized to achieve this goal, but such approaches using endogenous potato genes have rarely been applied. Since unpredictable global weather changes cause more severe and frequent water limiting conditions, improvement of potato drought tolerance can minimize such adverse effects under drought and can impact on sustainable potato production. Genetic engineering can be utilized to improve potato drought tolerance, but such approaches using endogenous potato genes have rarely been applied. We were obtained AtbZIP28 gene transgenic potato plants. It is identified transcript levels at various stress conditions, polyethylene glycol (PEG), NaCl, abscisic ${\underline{acid}}$ (ABA). Also, For identification to regulate ER stress response genes in AtbZIP28 gene transgenic potato plant, we screened seven potato genes from RNA-seq analysis under TM treatment. Five and two genes were up- and down-regulated by TM, respectively. Their expression patterns were re-examined at stress agents known to elicit TM, DTT, DMSO and salt stress.

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