EDISON SW 활용 경진대회 논문집 (Proceeding of EDISON Challenge)
- 제4회(2015년)
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- Pages.70-75
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- 2015
Mutation Effects on FAS1 Domain 4 Related to Protein Aggregation by Molecular Dynamics Simulations and Solvation Free Energy Analysis
- Cho, Sunhee (Department of Chemistry, Sookmyung Women's University) ;
- Ham, Sihyun (Department of Chemistry, Sookmyung Women's University)
- 발행 : 2015.03.19
초록
Fasciclin 1 (FAS1) is an extracellular protein whose aggregation in cornea leads to visual impairment. While a number of FAS1 mutants have been studied that exhibit enhanced/decreased aggregation propensity, no structural information has been provided so far that is associated with distinct aggregation potential. In this study, we have investigated the structural and thermodynamic characteristics of the wild-type FAS1 and its two mutants, R555Q and R555W, by using molecular dynamics simulations and three-dimensional reference interaction site model (3D-RISM) theory. We find that the hydrophobic solvent accessible surface area increases due to hydrophobic core repacking in the C-terminus caused by the mutation. We also find that the solvation free energy of the mutants increases due to the enhanced non-native H-bonding. These structural and thermodynamic changes upon mutation contribute to understand the aggregation of these mutants.
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