Development of System-Wide Functional Analysis Platform for Pathogenicity Genes in Magnaporthe oryzae
- Park, Sook-Young (Dept. of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University) ;
- Choi, Jaehyuk (Dept. of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University) ;
- Choi, Jaeyoung (Dept. of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University) ;
- Kim, Seongbeom (Dept. of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University) ;
- Jeon, Jongbum (Dept. of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University) ;
- Kwon, Seomun (Dept. of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University) ;
- Lee, Dayoung (Dept. of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University) ;
- Huh, Aram (Dept. of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University) ;
- Shin, Miho (Dept. of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University) ;
- Jung, Kyungyoung (Dept. of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University) ;
- Jeon, Junhyun (Dept. of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University) ;
- Kang, Chang Hyun (Dept. of Plant Biology, University of Georgia) ;
- Kang, Seogchan (Dept. of Plant Pathology & Environmental Microbiology, The Pennsylvania State University) ;
- Lee, Yong-Hwan (Dept. of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University)
- Published : 2014.10.30
Abstract
Null mutants generated by targeted gene replacement are frequently used to reveal function of the genes in fungi. However, targeted gene deletions may be difficult to obtain or it may not be applicable, such as in the case of redundant or lethal genes. Constitutive expression system could be an alternative to avoid these difficulties and to provide new platform in fungal functional genomics research. Here we developed a novel platform for functional analysis genes in Magnaporthe oryzae by constitutive expression under a strong promoter. Employing a binary vector (pGOF1), carrying
Keywords
- the rice blast fungus;
- functional analysis;
- Agrobacterium tumefaciens-mediated transformation;
- over-expression;
- system development