Efficacy of Gene Transfer and Expression of Recombinanat Baculovirus Vector System

재조합 베큘로바이러스벡터의 유전자전달과 발현의 효과

Novel baculovirus vector systems including genes of polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD) were constructed. These recombinant baculovirus vector systems were transfected into diverse cells of 293T, HepG2, HFF, and Hur7 cells and compared the effects of gene transfer and expression of these vector systems with control vector. From the result, we confirmed that these recombinant baculovirus vector systems were more excellent than control vector in efficacy of gene transfer and expression.

polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) 유전자가 포함된 새로운 재조합 베큘로바이러스를 제조하였다. 본 재조합 베큘로바이러스 시스템은 293T, HepG2, HFF, Hur7 세포에 감염하여 시험하였고 재조합된 유전자의 전이와 유전자 발현을 대조 벡터시스템과 비교하였다. 본 연구로부터 새롭게 제작된 재조합 베큘로바이러스 시스템이 감염에 의한 유전자의 전달과 해당 유전자 발현에 있어서 대조 벡터시스템 보다 우수한 효과를 나타내었다.