한국진공학회:학술대회논문집 (Proceedings of the Korean Vacuum Society Conference)
- 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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- Pages.199-199
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- 2013
Preferential Killing of Human Lung Cancer Cell Lines with Mitochondrial Dysfunction by Non-Thermal Dbd Plasma
- Panngom, Kamonporn (Department of Plasma Bioscience and Display, Kwangwoon University) ;
- Baik, Ku Youn (Department of Plasma Bioscience and Display, Kwangwoon University) ;
- Nam, Min-Kyung (Department of Biomedical Sciences, The Catholic University of Korea) ;
- Rhim, Hyang-Shuk (Department of Biomedical Sciences, The Catholic University of Korea) ;
- Choi, Eun Ha (Department of Plasma Bioscience and Display, Kwangwoon University)
- 발행 : 2013.02.18
초록
The distinctive cellular and mitochondrial dysfunctions of a human epithelial lung cancer cell line (H460) from a human lung fibroblastic normal cell line (MRC5) have been studied by dielectric barrier discharge (DBD) plasma treatment. The DBD plasma device have generated large amount of H2O2 and NOx in culture media which is dependent on plasma exposure time. It is found that the cell number of lung cancer cell H460 has been reduced more than the lung normal cell MRC5 as being increased exposure and incubation time. Also these both cell lines have showed mitochondria fragmentation under 5 minutes' plasma exposure, which is a clue of apoptosis. It is noted in this study that AnnexinV staining has showed not only early apoptosis, but also late apoptosis in lung cancer cell H460. Mitochondria enzyme activity and ATP generation have been also much reduced in lung cancer cell H460. Their mitochondrial membrane potential (