Proceedings of the Plant Resources Society of Korea Conference (한국자원식물학회:학술대회논문집)
- 2011.10a
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- Pages.14-14
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- 2011
Enhancement of Spermidine Content and Antioxidant Capacity by Modulating Ginseng Spermidine synthase in Response to Abiotic and Biotic Stresses
- Parvin, Shohana (Department of Oriental Medicinal Materials and Processing, College of Life Science, Kyung Hee University) ;
- Lee, Ok-Ran (Department of Oriental Medicinal Materials and Processing, College of Life Science, Kyung Hee University) ;
- Sathiyaraj, Gayathri (Department of Oriental Medicinal Materials and Processing, College of Life Science, Kyung Hee University) ;
- Kim, Yu-Jin (Department of Oriental Medicinal Materials and Processing, College of Life Science, Kyung Hee University) ;
- Khorolragchaa, Altanzul (Department of Oriental Medicinal Materials and Processing, College of Life Science, Kyung Hee University) ;
- Yang, Deok-Chun (Department of Oriental Medicinal Materials and Processing, College of Life Science, Kyung Hee University)
- Published : 2011.10.21
Abstract
Polyamines (putrescine, spermidine and spermine) play pivotal roles in plant defense to different abiotic and biotic stresses. In order to understand the function of ginseng spermidine synthase gene, a key gene involved in biosynthesis of polyamines, transgenic plant was generated in Arabidopsis. The transgenic plants exhibited high levels of polyamines compared to the untransformed control plants. We investigated the tolerance capacity of transgenic plants to abiotic stresses such as salinity and copper stress. In addition, transgenic plants also showed increased resistance against one of the important fungal pathogens of ginseng, the wilt causing Fusarium oxysporum and one of important bacteria, bacterial blight causing Pseudomonas syringae. However, an activity of the polyamine catabolic enzyme, diamine oxidase (DAO) was increased significantly in F. oxysporum and P. syringae infected transgenic plant. Polyamine catabolic enzymes which may trigger the hypersensitive response (HR) by producing hydrogen peroxide (
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