Proceedings of the Plant Resources Society of Korea Conference (한국자원식물학회:학술대회논문집)
- 2010.10a
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- Pages.11-11
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- 2010
Development of SSR markers for genetic mapping of Korean ginseng and authentication of Korean ginseng cultivars
- Kim, Nam-Hoon (Department of Plant Science, Seoul National University) ;
- Choi, Hong-Il (Department of Plant Science, Seoul National University) ;
- Jung, Ju-Yeon (Department of Plant Science, Seoul National University) ;
- Choi, Beom-Soon (National Instrumentation Center for Environmental Management) ;
- Ahn, In-Ok (KT&G Central Research Institute) ;
- Lee, Joon-Soo (KT&G Central Research Institute) ;
- Yang, Tae-Jin (Department of Plant Science, Seoul National University)
- Published : 2010.10.07
Abstract
The Korean ginseng, Panax ginseng C. A. Meyer is a popular medicinal herb in Araliaceae. Genetic map in crops provides valuable information for breeding, genetic and genomic researches. However, little information is available for construction of genetic map in ginseng. Up to now, we have produced large amounts of expressed sequence tags (ESTs) from four ginseng cultivars (37Mb, 49Mb, 39Mb, 47Mb from Gopoong, Gumpoong, Chunpoong and Yunpoong respectively using pyrosequencing technique and 5Mb from normalized full-length cDNA library of Chunpoong) to obtain comprehensive information of gene expression, and constructed EST database including ESTs from public database. Till now, we designed 261 SSR primer sets using EST sequences and identified 106 intergenic polymorphic markers. And 44 of the 106 showed polymorphisms among panax ginseng cultivars. Among 44 markers, 27 SSR polymorphic markers were inspected to 51