Proceedings of the KSME Conference (대한기계학회:학술대회논문집)

The Korean Society of Mechanical Engineers (대한기계학회)
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Effect of complex mechanical stimuli for MC3T3-E1 cells

MC3T3-E1 세포에 대한 복합 기계적 자극의 영향

  • 강경신 (포항공과대학교 기계공학과) ;
  • 이승재 (충남대학교 BK21 메카트로닉스 사업단) ;
  • 조동우 (포항공과대학교 기계공학과)
  • Published : 2008.11.05
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Abstract

The purpose of this study was to examine the effects of various mechanical stimuli for MC3T3-E1 cells. Among the several mechanical stimulations, we focused on compressive stain and ultrasound. In this study, we developed a bioreactor capable of applying controlled stimuli to scaffolds. PLLA/PCL scaffold was fabricated by using salt-leaching method. We performed dynamic cell culture using preosteoblasts MC3T3-E1 cells with 1MHz, 30mW/cm2 ultrasound and 10% of compressive strain. Result of CCK-8 analysis at 1, 4, 7, 10 days showed that mechanical stimuli had no significant effect for cell proliferation. However, those stimuli influenced ALP(Alkaline phopatase) activity, which is one of differentiation marker.

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