Proceedings of the KSME Conference (대한기계학회:학술대회논문집)
- 대한기계학회 2008년도 추계학술대회A
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- Pages.1690-1691
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- 2008
Micro-bioreactor for Physical stimulation of endothelial cells using micro-bead impact by gravitational force
미세입자의 중력을 이용한 세포 자극기 개발에 관한 연구
- 발행 : 2008.11.05
초록
Micro cell stimulation device is interested in many researchers because it has several advantages such as saving time and reagents. We introduce new micro-bioreactor using micro bead and conduct cell stimulation experiments to verify effective time because cell have operated by cell-cycle (G1, S, G2, and M phase). Micro-bioreactor was made by soft lithography and CAPE (calf pulmonary artery endothelial cell) was cultured in PDMS (polydimethylsiloxane) micro device for 12 hour and cell starvation process was performed for 24 hours. Micro glass beads were rolled only by slating device every hour during 15 hour because of minimizing other stimulation force like flow and pressure. The result represents that cells under exposed under micro bead stimulation show higher growth rate than normal condition and earlier and later stimulation time are more effective.