한국전기전자재료학회:학술대회논문집 (Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference)

한국전기전자재료학회 (The Korean Institute of Electrical and Electronic Material Engineers)
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비수식화 DNA를 이용한 차세대형 바이오칩의 개발

Development of Next Generation Biochip Using Indicator-free DNA

  • Choi, Yong-Sung (Department of Electrical Engineering, DongShin University) ;
  • Moon, Jong-Dae (Department of Electrical Engineering, DongShin University) ;
  • Lee, Kyung-Sup (Department of Electrical Engineering, DongShin University)
  • 발행 : 2006.05.19
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초록

This research aims to develop a multiple channel electrochemical DNA chip using micro- fabrication technology. At first, we fabricated a high integrated type DNA chip array by lithography technology. Several probe DNAs consisting of thiol group at their 5-end were immobilized on the gold electrodes. Then target DNAs were hybridized by an electrical force. Redox peak of cyclic-voltammogram showed a difference between target DNA and mismatched DNA in the anodic peak current. Therefore. it is able to detect a various genes electrochemically after immobilization of a various probe DNA and hybridization of label-free DNA on the electrodes simultaneously. It suggested that this DNA chip could recognize the sequence specific genes.

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