Electrochemical Gene Detection Using Hoechat Groove Binder

Hoechst groove binder를 이용한 유전자의 전기화학적 검출

  • Choi, Yong-Sung (Department of Electrical Engineering, DongShin University) ;
  • Lee, Woo-Ki (Department of Electrical Engineering, DongShin University) ;
  • Lee, Kyung-Sup (Department of Electrical Engineering, DongShin University)
  • Published : 2006.05.19

Abstract

In this study, a DNA chip with a microelectrode array was fabricated using microfabrication technology. Several probe DNAs consisting of mercaptohexyl moiety at their 5 end were immobilized on the gold electrodes by DNA arrayer. Then target DNAs were hybridized and reacted with Hoechst 33258, which is a DNA minor groove binder and electrochemically active dye. Linear sweep voltammetry or cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. It was derived from Hoechst 33258 concentrated at the electrode surface through association with formed hybrid. It suggested that this DNA chip could recognize the sequence specific genes.

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