한국생물공학회:학술대회논문집
- 2005.10a
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- Pages.651-654
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- 2005
Analysis of intracellular amino acids in the fermentation of Pichia pastoris X-33 and KM71H
- Han, Kyung-Ah (Department of Material and Biochemical Engineering, BioProcess Technology lab., Research Center for BioPhotonics, Chonnam National University) ;
- Kim, Sun-Yong (Faculty of Applied Chemical Engineering, BioProcess Technology lab., Research Center for BioPhotonics, Chonnam National University) ;
- Rhee, Jong-Il (Faculty of Applied Chemical Engineering, BioProcess Technology lab., Research Center for BioPhotonics, Chonnam National University)
- Published : 2005.10.27
Abstract
Analysis of extra- and intra- cellular metabolites is very important to study cell metabolish. Intracellular amino acids in yeast are of great interest as precursors of desired products in biomass production of the feed industries and the production of glutamate. In this study, the fermentations of P. pastoris X-33 and KM71H were carried out in shake flasks. After centrifugation, the harvested cells were mechanically disrupted by using glass bead. The supernatants were used to analyze intracellular amino acid by HPLC system. For HPLC analysis, Resolve C18 column was used with Fluorescence detector. The OPA(o-phthalaldehyde) derivation reaction was employed for analysing amino acids at 30
10 g/L yeast extract, 20 g/L peptone, 20 g/L dextrose가 첨가된 YPD 배지를 이용하여 200 rpm, 30
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