한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characterization of novel EGCG (Epigallocatechin gallate) analogues synthesized by glucansucrase from Leuconostoc mesenteroides B-1299CB

  • Moon, Young-Hwan (Department of Material Chemical and Biochemical Engineering) ;
  • Lee, Jin-Ha (Engineering Research Institute) ;
  • Nam, Seung-Hee (School of Biological Sciences and Technology, Chonnam National University) ;
  • Kim, Do-Won (Kangnung National University) ;
  • Oh, Deok-Kun (Department of Bioscience and Biotechnology, Sejong University) ;
  • Kim, Do-Man (School of Biological Sciences and Technology, and The Research Institute for Catalysis, Institute of Bioindustrial Technology, Chonnam Natioanl University, Biology Research Center for Industrial Accelerator, Dongshin University)
  • Published : 2005.10.27
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Abstract

Three EGCG analogues were synthesized by the acceptor reaction of a glucansucrase from Leuconostoc mesenteroides B-1299CB with EGCG and sucrose. The transfer products was purified using Sephadex LG-20 column chromatography and high performance liquid chromatography (HPLC). EGCG-G1 and EGCG-G2 were novel compounds for the first time reported in this paper. EGCG glycosides showed similar or slower antioxidative effects according to their structures $(EGCG{\geq}EGCG-G1>EGCG-G1'>EGCG-G2)$. However, the water solubilities of the EGCG-G1, EGCG-G1' and EGCG-G2 were 52, 76 and 140 times higher than that of EGCG. Furthermore, they showed more browning resistance against UV irradiation than EGCG.

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