한국생물공학회:학술대회논문집
- 한국생물공학회 2005년도 생물공학의 동향(XVI)
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- Pages.420-422
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- 2005
Strain Improvement and Genetic Characterization of Tautomycetin Biosynthesis in Streptomyces spp.
- Choi, Si-Sun (ForHuman Tech co., Ltd., Suwon University) ;
- Kim, Myung-Gun (Department of Biological Engineering, Inha university) ;
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Kim, Eung-Soo
(Department of Biological Engineering, Inha university)
- 발행 : 2005.04.15
초록
TMC (Tautomycetin) is a liner polyketide immunosuppressive antifungal compound produced by Streptomyces spp. Inhibition of T cell proliferation with TMC was observed highly efficient at 100-fold lower than those needed to achieve maximal inhibition with cyclosporin A. To elucidate the biosynthetic pathway of TMC, a genomic DNA library was constructed using a E. coil-Streptomyces shuttle cosmid vector, pOJ446. The DNA libraries were screened by colony blot hybridization using several polyketide
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