한국생물공학회:학술대회논문집
- 2005.04a
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- Pages.396-397
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- 2005
Isolation of Novel Pseudonocardia Polyene Biosynthetic Genes via Genomics-based PCR Screening
- Lee, Mi-Yeon (Department of Biotechnology, Inha University) ;
- Hwang, Young-Bin (Department of Biotechnology, Inha University) ;
- Park, Hyun-Joo (SongWon Envichem Inc.) ;
- Han, Kyu-Boem (Hanson Biotech Co., Ltd. 201 IACRI, Han Nam Univ.) ;
- Kim, Eung-Soo (Department of Biotechnology, Inha University)
- Published : 2005.04.15
Abstract
The polyene antibiotics are a family of most promising antifungal polyketide compounds, typically produced by actinomycetes species. Using the polyene CYP-specific PCR screening with served actinomycetes genomic DNAs, Pseudonocardia autotrophica strain was identified to contain a unique polyene-specific CYP gene. The genomic DNA library screening using the polyene-specific CYP gene probe revealed the positive cosmid clone containing an approximately 34.5 kb DNA fragment revealed a total of seven complete and two incomplete open reading frame (ORFs), which are highly homologous but unique to previously-known polyene biosynthetic genes. These results suggest that the polyene-specific screening approach should be an efficient way of isolating potectially-valuable cryptic polyene biosynthetic gene cluster from various rare actinomycetes.
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