한국생물공학회:학술대회논문집
- 한국생물공학회 2005년도 생물공학의 동향(XVI)
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- Pages.68-72
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- 2005
Statistical Optimization of Medium Composition for Growth of Leuconostoc citreum
- Kim, Hyun (Department of Food Science and Technology, Chungbuk National University, Research Center for Bioresource and Health) ;
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Eom, Hyun-Ju
(Department of Food Science and Technology, Chungbuk National University, Research Center for Bioresource and Health) ;
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Lee, Jun-Soo
(Department of Food Science and Technology, Chungbuk National University, Research Center for Bioresource and Health) ;
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Seo, Jin-Ho
(Department of Food Science and Technology, Seoul National University) ;
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Han, Nam-Soo
(Department of Food Science and Technology, Chungbuk National University, Research Center for Bioresource and Health)
- 발행 : 2005.04.15
초록
Leuconostoc citreum is one of the representative strains of Leuconostoc spp. that show fast growth rates in fermented vegetables. Sequential experimental designs including the Plackett-Burman design, fractional factorial design, steepest ascent analysis, central composite design and response surface methodology were introduced tooptimize and improve the medium for Leuconostoc citreum. Fifteen medium ingredients were examined and glucose (20 g/l), yeast extract (12.5 g/l), sodium acetate trihydrate (6.12 g/l), potassium phosphate (42.55 g/l) and dibasic ammonium citrate (4.12 g/l)were chosen as the best components to give a critical and positive effect for cell-growth. The biomass was increased to 2.79 g/l (169%), compared to the 1.65 g/l in MRS medium.
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