Proceedings of the Korean Environmental Health Society Conference (한국환경보건학회:학술대회논문집)

Korean Society of Environmental Health (한국환경보건학회)
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Heme Oxygenase-1(HO-1) induction by UVB(290-320nm) radiation in ICR mice

  • Choi, Wook-Hee (Department of Health science, Dongduk women's University) ;
  • Kim, Tae-Hwan (College of Veterinary medicine, Kyungbook national University) ;
  • Ahn, Ryoung-Me (Department of Health science, Dongduk women's University)
  • Published : 2005.11.04
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Abstract

The induction of heme oxygenase-1(HO-1) by ultraviolet(UV) radiation provides a protective defense against oxidative stress, and has been well demonstrated in UVA-irradiated skin, but not UVB. In this study in mice, we show that the UVB(290-320nm) radiation can be attributed to the induction of cutaneous heme oxygenase-1. The expression of HO-1 mRNA was assessed in vivo by the reverse transcription-polymerase chain reaction (RT-PCR) analysis, and HO-1 enzyme activity was measured in microsomal preparation from irradiated mice. The mRNA level of HO-1 increases in liver and skin from 24h to 72h after UVB($3KJ/m^3$) radiation. The results of gene expression were same pattern of HO enzyme activity in skin, but not in liver. HO-1 mRNA in liver resulted in a progressive increase to 96h after UVB radiation, but HO activity in liver increased to 48h. This finding indicates that UVB radiation is an important inducer of HO-1 and increases in HO activity may protect tissues directly or indirectly from oxidative stress.

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