Proceedings of the Korean Environmental Health Society Conference (한국환경보건학회:학술대회논문집)
- 2005.06a
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- Pages.337-340
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- 2005
A study on Determination Method of (N-2-hydroxy-ethyl)valine(HEV) in Hemoglobin Adducts for Biological Monitoring of Ethylene Oxide Exposure
- Lee, Jin-Heon (Department of Environmental Education, Kongju National University) ;
- Shin, Ho-Shang (Department of Environmental Education, Kongju National University) ;
- Ahn, Hye-Sil (Department of Environmental Education, Kongju National University)
- Published : 2005.06.03
Abstract
Ethylene oxide is a genotoxic carcinogen with widespread uses as industrial chemical intermediate and gaseous sterilant. 2-hydroxyethylated N-terminal valine in Hb is a good biomarker for biological monitoring of ethylene oxide exposure, because of its stability. We studied the determination method of (N-2-hydroxy-ethyl)valine in hemoglobin adduct by using GC/MS. PFPITC and TBMS were used as appropriate derivatives. Ethylene oxide formed Hb adducts as (N-2-hydroxy-ethyl)valine(HEV) in mouse with ethylene oxide inhalation exposure. Standard HEV can be synthesized with 2-amino-ethanol and 2-bromo-3-methylbutyric acid. GC/MS can measured them after derivatization with pentafluorophenylisothiocianate(PFPITC) and N-(tertiary butyl dimethylsiiyl)-N-methyl-trifluoroacetamide(TBDMS-TFA) by using Edman procedure. Concentrations of Hb adduct were proportionally increased with exposure levels. They were 230
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