Endocrine Disrupting Effects of Several Pharmaceuticals to Oryzias Latipes

Endocrine disrupting effects of four pharmaceutical products were evaluated with fish. The test pharmaceuticals, i.e., sulfamethoxazole, sulfamethazine, oxytetracycline and tetracycline have been often detected in aquatic environment, but their ecological hazard on receptors of various trophic levels has seldom been evaluated. In the present study, we conducted acute toxicity assays with a fish, Japanese medake (Oryziα lαtipes). The vitellogenin induced in female fish normally, but a endocrine disrupting chemical could give effects even male fish. We have tried 4 pharmaceutic chemicals to find out the endocrine disrupting effects. Sulfamethoxazole 1, 0.5 ppm induced vitellogenin even at male Japanese medaka. Sulfamethazine 10, 5, 1 ppm could induced vitellogenin at male fish. Oxytetracycline 10, 5, 1ppm could induced vitellogenin With the fish. Tetracycline 10, 5 ppm could induced vitellogenin at male fish. Some pharmaceuticals such as sulfamethoxazole, sulfamethazine, oxytetracycline and tetracycline could give effects to male Oryzias latipes. They could induced vitellogenin under exposure range 0.5 ${\sim}$ 10 ppm of chemicals at male Oryzias latipes.