Modulating Effects on Vitellogenin Induction by Several Pharmaceuticals to Oryzias latipes

Endocrine disrupting effects of several pharmaceutical products were evaluated with fish. The test pharmaceuticals(caffeine, ketoconazole, acetaminophen and diltiazem) have been often detected in aquatic environment of Korea(from on going study of this research team). We analyzed vitellogenin induction by qualitative (Western blot) and quantitative (ELISA) assay. $17{\beta}$ -estradiol was used as a positive control. Some pharmaceuticals could give effects to male Oryzias latipes. They could induced vitellogenin under exposure of chemicals at male Oryzias latipes.