한국식품영양과학회:학술대회논문집 (Proceedings of the Korean Society of Food Science and Nutrition Conference)
- 한국식품영양과학회 2004년도 Annual Meeting and International Symposium
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- Pages.53-60
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- 2004
Effects of Isothiocyanates on Antioxidant Response Element-mediated Gene Expression and Apoptosis
- Hong Sung-Jae (Department of Biochemistry, School of Medicine, Wonkwang University) ;
- Kim Sung-Min (Department of Biochemistry, School of Medicine, Wonkwang University) ;
- Kim Young-Sook (Department of Biochemistry, School of Medicine, Wonkwang University) ;
- Hu Rong (Department of Pharmaceutics, Ernest Marie School of Pharmacy, Rutgers University) ;
- Kong A.N. Tony (Department of Pharmaceutics, Ernest Marie School of Pharmacy, Rutgers University) ;
- Kim Bok-Ryang (Department of Biochemistry, School of Medicine, Wonkwang University)
- 발행 : 2004.11.01
초록
The pro-apoptotic effect of phenethyl isothiocyanate (PEITC) and the role of glutathione (GSH) in sulforaphane (SFN)-induced antioxidant response element-dependent gene expression were investigated. The caspase-3 and caspase-9 activities were stimulated by PEITC. The release of cytochrome c was time- and dose- dependent. SP600125 suppressed apoptosis induced by PEITC. Similarly, this JNK inhibitor attenuated both cytochrome c release and caspase-3 activation induced by PEITC. SFN is converted to the glutathione conjugate by glutathione S-transferases (GSTs). It was accumulated in mammalian cells by up to several hundred-fold over the extracellular concentration, by conjugation with intracellular GSH. The induction of ARE by SFN was 8.6-fold higher than by SFN-NAC. The decrease in ARE expression at higher concentrations of SFN and SFN-NAC was correlated with the accelerated apoptotic cell death, with a dose-dependent activation of caspase 3 activity by SFN. Upon addition of extracellular GSH within 6 hr of treatment with SFN, the effect on ARE expression was blocked almost completely.
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