Genetic Differences within and between Populations of Korean Catfish (S. asotus) and Bullhead (P. fulvidraco) Analysed by RAPD-PCR

Of the 20 arbitrarily chosen primers, six oligonucleotides decamer primers were used on the basis of the number of the polymorphisms generated in catfish (Silurus asotus) from Yesan and bullhead (Pseudobagrus fulvidraco) from Dangjin in Korea. Six primers were used generating a total of 602 scorable bands in catfish and 195 in bullhead population, respectively, ranging in size of DNA fragments from less than approximately 100 to larger than 2,000 base pairs (bp). Six primers yielded 199 polymorphic fragments (33.1 %) in catfish and 47 (24 %) in bullhead, respectively. In the present study, a total of 328 common fragments (an average of 54.7 per prime.) were observed in catfish population, whereas 84 (an average of 14.0 per prime.) in bullhead. The total number of specific fragments in catfish and bullhead population were 76and 64, respectively.