Proceedings of the Korea Society of Poultry Science Conference (한국가금학회:학술대회논문집)

The Korean Society of Poultry Science (한국가금학회)
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Development of novel markers for the characterization of chicken primordial germ cells

  • Lee, Bo-Ram (Division of Animal Genetic Engineering, School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Duk-Kyung (Division of Animal Genetic Engineering, School of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Young-Mok (Division of Animal Genetic Engineering, School of Agricultural Biotechnology, Seoul National University) ;
  • Jung, Jin-Gyoung (Avicore Biotechnology Institute Inc.) ;
  • Kim, Jin-Nam (Avicore Biotechnology Institute Inc.) ;
  • Lee, Seon-Duk (Avicore Biotechnology Institute Inc.) ;
  • Park, Tae-Sub (Division of Animal Genetic Engineering, School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University) ;
  • Lim, Jeong-Mook (Division of Animal Genetic Engineering, School of Agricultural Biotechnology, Seoul National University) ;
  • Han, Jae-Yong (Division of Animal Genetic Engineering, School of Agricultural Biotechnology, Seoul National University)
  • Published : 2004.11.01
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Abstract

We developed a new panel of markers for the characterization of chicken PGCs. The results of immunostaining demonstrated that anti-SSEA-3, anti-SSEA-4, anti-integrin 6, and anti-integrin 1 antibodies. and STA and DBA bound specifically to chicken PGCs. These reagents could be used to characterize chicken PGCs together with conventional marker reagents such as PAS and anti-SSEA-1 antibody. We also showed that double staining of PGCs with the newly developed markers was feasible, which might contribute to rapid detection and accurate characterization of chicken PGCs.

형질전환 가금의 생산은 생체반응기(Bioreactor)가금에 의한 고부가가치의 생의약 물질을 저비용, 고효율로 생산할 수 있으며, 배 발달과정 및 유전자 조절기작 규명을 통한 학문적 이용성 등 다양한 분야에 응용될 수 있다. 형질전환 가금을 생산하기 위한 방법 중 닭의 배 발생 초기에 발생하는 성세포(정자 혹은 난자)의 전구세포인 원시생식세포를 이용한 연구가 활발하게 진행되고 있다. 그러나 이를 검증할 원시생식세포 특이적 마커의 부재로 많은 어려움을 겪고 있다. 따라서 본 연구는 원시생식세포의 특성 분석을 위해 PAS(Periodic acid-Schiff) 염색 및 특이항체 (SSEA-1,3,4 & Integrins $\alpha$6, $\beta$l) 그리고 lectins (STA, DBA, ConA, WGA)를 이용하였다. 이번 연구결과를 통한 닭 원시생식세포의 특이적 마커의 개발은 원시생식세포를 이용한 가금의 형질전환 연구에 기여할 것이다.

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