Toluene Monooxygenase의 Peroxide shuntting에 의한 TCE와 PCE 분해 특성

  • 류두현 (전주대학교 과학기술부 환경시스템전공) ;
  • 김형수 (전주대학교 과학기술부 환경시스템전공) ;
  • 최용욱 (전주대학교 과학기술부 화학전공) ;
  • 김용미 ((주)동명엔터프라이즈) ;
  • 이경애 (전주대학교 과학기술부 환경시스템전공) ;
  • 유재수 (전주대학교 과학기술부 환경시스템전공) ;
  • 조현 (전주대학교 과학기술부 환경시스템전공)
  • Published : 2004.09.01

Abstract

TCE and PCE, suspected carcinogens, are the most common groundwater pollutant from extensive use as a solvent and degreaser. Escherichia coli TGI pBSKAN TOM Green and E. coli TGI pBSKAN ToMO, which were used DNA shuffling technique, produce Toluene-o-monooxygenase(TOM) and toluene-o-xylene- monooxygenase(ToMO). These cells and enzymes are degrading TCE and PCE, TOM and ToMO are needed to cofactor, such as NADH, NADPH and other cofactors. Used TCE and PCE degrading microorganisms experiment the contaminated material removal efficiency. A shunting test used NAD and Hydrogen peroxide.

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