Proceedings of the Membrane Society of Korea Conference (한국막학회:학술대회논문집)

The Membrane Society of Korea (한국막학회)
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Extraction behavior of $\alpha$-lactalbumin using reverse micellar system

  • Noda, Kazuki (Department of Chemical Engineering, Nara National College of Technology) ;
  • Konishi, Taiji (Department of Chemical Engineering, Nara National College of Technology) ;
  • Naoe, Kazumitsu (Department of Chemical Engineering, Nara National College of Technology) ;
  • Kawagoe, Mikio (Department of Chemical Engineering, Nara National College of Technology) ;
  • Imai, Masanao (Department of Science ＆ Technology, College of Bioresource Sciences, Nihon University)
  • Published : 2004.05.01
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Abstract

This study reports the extraction behavior of $\alpha$-lactalbumin using bis(2-ethylhexyl) sulfosuccinate sodium (AOT) reverse micelles. Forward extraction of $\alpha$-lactalbumin in the reverse micellar organic phase from aqueous feed solutions was strongly dependent on the AOT concentration and the complete forward extraction of 0.03 mM $\alpha$-lactalbumin was successfully achieved at an AOT concentration of ca. 100 mM. A similar dependency of the forward extraction on the AOT concentration was obtained in isooctane, n-hexane, and n-octane systems. In the backward extraction from the micellar organic phase, the recovery of the protein as high as ca. 90% was obtained with pH control and/or salt addition.

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