Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2004.06a
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- Pages.260-260
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- 2004
Calcium Signaling of M II Oocyte during Chemical Activation of Calcium Ionopore and Cytochalasin B
- Kim, Sung-Woo (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
- Park, Jin-Ki (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
- Park, Chun-Gyu (Division of Animal Biotechnology, National Livestock Research Institute, RDA, Department of Animal Science, Graduate School, Konkuk University) ;
- Lee, Ju-Young (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
- Han, Joo-hee (Division of Animal Biotechnology, National Livestock Research Institute, RDA, Department of Animal Science, Graduate School, Konkuk University) ;
- Lee, Seung-Eun (Division of Animal Biotechnology, National Livestock Research Institute, RDA, Department of Animal Science, Chungbuk National University) ;
- Baek, Kyung-Nye (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
- Chang, Won-Kyung (Division of Animal Biotechnology, National Livestock Research Institute, RDA)
- Published : 2004.06.01
Abstract
The calcium ionophore (A23187) has been used for activation of porcine oocytes from in vitro maturation by many researches. The signaling of calcium was known to be a primary factor of activation of MII oocyte by calcium ionophore. The calcium level was measured by an intensity of fluo 4 fluorescence and confocal microscope. The level was increased by 7% ethanol or 70 μM calcium ionophore but oscillation was not found. (omitted)