한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Solvent-tolerance and trehalose accumultion by expression of otsA and otsB homologs in the response to toluene of Pseudomonas sp. BCNU 106 isolated from waste water

  • Bae, Yun-Ui (Institute of Genetic Engineering, Changwon National University) ;
  • Park, Hyeong-Cheol (Institute of Genetic Engineering, Changwon National University) ;
  • Yoo, Ju-Soon (Institute of Genetic Engineering, Changwon National University) ;
  • Kim, Ki-Wook (Institute of Genetic Engineering, Changwon National University) ;
  • Cho, Soo-dong (Institute for Basic Science, Changwon National University) ;
  • Moon, Ja-Young (Changwon National University) ;
  • Jeong, Yong-Kee (Department of Microbiology, Dong-Eui University) ;
  • Joo, Woo-Hong (Department of Biology, Changwon National University)
  • Published : 2003.10.22
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Abstract

Pseudomonas sp. BCNU 106 accumulated approximately 4.12 mM trehalose after cultivation of 12 hr probably by the arising action of trehalose-6-phosphate synthase/phosphatase. The cDNA clones of trehalose-6-phosphate synthase/ phosphatase were isolated from Pseudomonas sp. BCNU 106, and named as PsTPS and PsTPP(Pseudomonas sp. BCNU 106 trehalose-6-phosphate synthase/phosphatase). The two mRNA levels of trehalose-6-phosphate synthase/ phosphatase peaked at 12 hr after exposure to toluene, and thereafter were declined slightly These results support an important role of trehalose accumulation by expressions of PsTPS and PsTPP in toluene-tolerance of Pseudomonas sp. BCNU 106.

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