Nano-Scale Immobilization of Antibody for the Construction of Immunosensor

Performance of an immunosensor can usually be assessed in terms of its analytical sensitivity and specificity. Sensitivity, i.e., the detection limit of analyte, is particularly determined by the amount of analyte molecules bound to the capture antibody immobilized onto a solid surface. In order to increase the binding complexes, we have investigated an immobilization method of antibody allowing for a molecular arrangement of the protein on a selective surface of a nano-patterned solid substrate. This has not been accomplished only by a surface treatment with a chemical, but also by fragmentation of immunoglobulin. Such approach would offer a protocol of antibody immobilization for the construction of nano-immunosensor and eventually improve the sensitivity of detection.