한국생물공학회:학술대회논문집
- 한국생물공학회 2003년도 생물공학의 동향(XIII)
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- Pages.480-484
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- 2003
Enhanced thermostability and substrate susceptibility of esteraseusing directed evolution
- 발행 : 2003.10.22
초록
A stereoselective-hydrolysing enzyme was cloned from Pseudomonas fluorescens KCTC1767 which had high enantiomeric activity toward (S)-ketoprofen ethyl ester. Analyses of typical properties resulted in low thermostability and substrate specificity. A round error-prone PCR and StEP(STaggered Extension Process) was adopted to evolute this character. As a result, the best clone 6-52 was selected which was represented to increased thermostability(40 fold) compared to wild type enzyme in
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