Application of a Dermal Equivalent to Organ Culture of Human Scalp Hair Follicle

The recent development of methods for culturing hair follicles in vitro has proved an important tool to investigate many aspects of drug screening. Human hair follicle is composed of multiple types of cells, whose interactions regulate morphology and cycling-anagen, catagen, and telogen. Many investigators have tried to develop models to prolong of the period of hair elongation in vitro. However these are limited in submerged culture, which don't work due to the lack of cell-cell interactions which are abundant in vivo environment. So we applied dermal equivalent (DE) to culturing flair follicles to prolong hair growth period.

본 연구는 in vivo 와 유사한 환경을 조성하여 기존의 기관 배양기간인 7일보다 연장하려는 연구이며 세포가 있는 경우 세포가 없는 경우보다 morphology나 길이 성장 면에서도 모두 우수한 결과를 나타냈으며 minoxidil 첨가실험을 통해 DE를 이용한 모델이 단순 액침배양보다 한 단계 진보된 hair growth model 임을 확인할 수 있었다.