한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Kidney reconstruction using kidney cell transplantation in kidney failure animal model

  • Kim, Sang-Soo (Department of Chemical Engineering, Hanyang University, Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University) ;
  • Park, Heung-Jae (Department of Urology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine) ;
  • Han, Joung-Ho (Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Choi, Cha-Yong (Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, School of Chemical Engineering, Seoul National University) ;
  • Kim, Byung-Soo (Department of Chemical Engineering, Hanyang University)
  • Published : 2003.10.22
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Abstract

Dialysis and renal transplantation, the current therapies for end-stage renal disease (ESRD), have many limitations including severe complications, organ shortage, and graft failure. To overcome the limitations, the present study investigated the reconstruction of renal tissue in vivo by transplanting isolated fetal renal cells using fibrin gel to the kidney of renal failure rat model. After 4 weeks from the transplantation, blood urea nitrogen(BUN) and creatinine were examined from blood samples and histological examination of the implanted tissues revealed formation of renal-like structures and restoration of renal function.

본 연구에서는 신장세포를 이용하여 신장을 재생하는 조직공학적인 신장 재생 방법을 개발하기 위해 신생 rat으로부터 분리한 신장세포를 피브린 고분자와 혼합하여 신부전 rat의 신장에 주사 이식하였고 4주 후에 신장 구조의 형성 및 개선된 BUN, creatinine 수치를 확인하였다. 이는 이식된 세포에 의해 신부전의 증상이 완화(치료)된 것으로, 앞으로 이에 대한 추가적인 장기간의 실험이 필요하다.

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