한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Optimization of media compositions on carbohydrolase complex production in liquid culture of Lentinus edodes

  • Park, Jeom-Seok (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Choi, Mim-Gu (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Ji, Young-Min (Division of Biotechnology and Genetic Engineering, Korea University) ;
  • Choi, Jeong-Woo (Chemical Engineering, Sogang University) ;
  • Hong, Eock-Kee (School of Biotechnology and Bioengineering, Kangwon National University)
  • Published : 2003.10.22
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Abstract

This study was concentrated on investigating the optimum compositions of medium for the production of carbohydrolases complex. In order to maximize the activities of the carbohydrolases complex, cellulose and yeast extract were chosen as the best C and N sources, respectively. The concentrations of cellulose and yeast extract as well as minerals and amino acids were also studied. On the other hand, ${\beta}-glucosidase$ and ${\beta}-glucuronidase$ showed the maximal activities at $60^{\circ}C$ and pH 4, while ${\beta}-galactosidase$ showed the maximal activity at $50^{\circ}C$ and pH 3.

본 연구는 고수율의 아라비녹실란을 얻기 위해 필수요소인 Lentinus edodes의 액체배양을 통해 생산되어지는 탄수화물 복합효소의 생산수율을 증대시키는데 그 목적이 있다. 그러기 위해 탄수화물 복합효소의 생산에 최적 배지성분을 검토한 결과 탄소원으로는 cellulose, 질소원으로는 yeast extract 가 선택되었다.

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