한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Study on recombinant expression of Phospholipase C gene (plc) in methylotrophic yeast Pichia pastoris and its properties

  • Seo, Kook-Hwa (Chonnam National University, Department of Materials and Biochemical Engineering, Bioprocess Technology Lab.) ;
  • Rhee, Jong-Il (Faculty of Applied Chemical Engineering, Bioprocess Technology Lab.)
  • Published : 2003.10.22
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Abstract

The phospholipase C (PLC) hydrolyzes the polar head groups such as phosphocholine or phosphoethanolamine residues esterified at the sn-3 position of phospholipids. Pichia pastoris can utilize methanol as a carbon source and produce recombinant proteins under the control of the strong, tightly-regulated alcohol oxidase (AOX) promoter. In this study, we developed recombinant P. pastoris system for PLC expression and analyzed PLC activity.

본 연구에서는 산업적으로 중요성이 날로 증가하고 있는 PLC의 대량 생산을 위해서 B. cereus ATCC10987에서 분리된 PLC 유전자를 pPICZa C에 삽입한 P. pastoris system을 개발하여 extracellular PLC를 생산하였다. 또한, 공업적 이용을 위해 여러 조건에서 B. cereus와 P. pastoris에서 생산된 extracellular PLC의 활성도를 측정하여 특성을 살펴보았다.

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