한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characterization of a thermostable fructosyltransferase from Leuconostoc mesenteroides TL1

  • 이광옥 (전남대학교 물질생물화학공학과) ;
  • 강희경 (전남대학교 공업기술연구소) ;
  • 김도만 (전남대학교 물질생물화학공학과, 전남대학교 응용화학공학부)
  • Published : 2003.04.11
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Abstract

A fructosyltransferase from Leuconostoc mesenteroides TL1 was purified. The molecular mass of the enzyme was estimated to be 38 kDa based on the activity staining. The pH and temperature optima of the enzyme were 6.3 and $40^{\circ}C$, respectivly. Structural analysis of the polymer prepared from sucrose by the enzyme was determined by NMR Spectroscopy: It shows the heterogeneous linkages of levan-like fructan and dextran-like glucan.

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