한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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PCA를 이용한 유전자 재조합 대장균의 ALA 생산공정의 해석

  • 강태형 (전남대학교, 산업공학과) ;
  • 정상윤 (전남대학교, 물질 생물화학공학과, 생물공정기술연구실) ;
  • 임용식 (전남대학교, 물질 생물화학공학과, 생물공정기술연구실) ;
  • 김춘광 (전남대학교, 물질 생물화학공학과, 생물공정기술연구실) ;
  • 정상욱 (전남대학교, 산업공학과) ;
  • 이종일 (전남대학교, 응용화학공학부, 생물공정기술연구실)
  • Published : 2003.04.11
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Abstract

ALA is an intermediate in the tetrapyrrole biosynthesis pathway and has extensive applications as a biodegradable herbicide and insecticide as well as medical applications including photodynamic therapy of cancers. For the development of mass production process of ALA it is necessary to on-line monitor some metabolites such as glycine, succinate, LA and ALA. In this study, medium compositions and fermentation conditions were investigated for enhancement of ALA production by recombinant E. coli. A 2-dimensional fluorescence sensor was employed to monitor the bioprocess of ALA production. The monitored data is analyzed using principal component analysis, a powerful tool for multivariate statistical analysis.

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