Production of Laccase and Bioremediation of Pentachlorophenol by Wood-Degrading Fungus Trichophyton sp. LKY-7 immobilized in Ca-Alginate Beads

  • 정현채 (순천대학교 농업생명과학대학) ;
  • 현규환 (순천대학교 농업생명과학대학) ;
  • 박종열 (경상대학교 산림과학부)
  • Published : 2003.04.24

Abstract

The lignin-degrading fungus Trichophyton sp. LKY-7 was immobilized in ca-alginate beads for laccase production and PCP remediation. The immobilized Trichopphyton sp. LKY-7 enabled the repeated use of this fungus for laccase production and produced high amount of laccase throughout 5 cycles incubation. As a laccase inducer oak wood meal(Quercus variabilis) seemed to be effective laccase inducer for Trichophyton sp. LKY-7, and the optimum addition amount was 1%(W/W) in glucose-peptone medium. Biotransformation of pentachlorophenol by immobilized Trichophyton sp. LKY-7 reached an efficency of up to 90% without toxic inhibition. Immobilized Trichophyton sp. LKY-7 might thus be applicable for semicontinuous laccase production and bioremediation to serve inoculum for reactor system.

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