Proceedings of the Korean Environmental Sciences Society Conference (한국환경과학회:학술대회논문집)
- 2003.11a
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- Pages.99-102
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- 2003
Analysis of Microtoxins in the Nakdong River Watershed
- Park, Jung-Min (Nakdong River Water Environment Laboratory, NIER, Ministry of Environment) ;
- Lee, Jae-Jung (Nakdong River Water Environment Laboratory, NIER, Ministry of Environment) ;
- Hwang, Dong-Jin (Nakdong River Water Environment Laboratory, NIER, Ministry of Environment) ;
- Yang, Sang-Yong (Nakdong River Water Environment Laboratory)
- Published : 2003.11.01
Abstract
The different methods such as HPLC, indirect- and direct-ELISA were employed for the analysis of microtoxins and the results of each method were compared in terms of the detection limit and accurary. Three toxins, microcystin-RR, -LR and -YR were clearly separated by HPLC using 0.05 M methanol and phosphate buffer used as a solvent system. The calibration curves for the toxins were linear in the range of 5 ng to 50 ng. The standard curves for the immunoassay of microcystin obtained by direct and indirect ELISA are compared. The linear responses of inhibitions of binding by microcystin in the direct and indirect competitive ELISA were in the range of 10 ng to 1000 ng and 50 pg to 160 pg, respectively. Distribution of microtoxins at 11 sites in the Nakdong river and several lakes in Korea was also studied. The most dominant microcystin variant in the test sites was found to be microcystin-RR.