대한약학회:학술대회논문집 (Proceedings of the PSK Conference)

대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Quantitative Analysis of Puerarin and Daidzein in Domestic and Imported Puerariae Radix by High Performance Liquid Chromatography

  • Kim, Hwa-Soon (Seoul Metropolitan Government Research Institute of Public Health and Environment Kangbuk Agro-Fishery Products Inspection center) ;
  • Park, Noh-Woon (Seoul Metropolitan Government Research Institute of Public Health and Environment Kangbuk Agro-Fishery Products Inspection cente) ;
  • Kang, Hee-Gon (Seoul Metropolitan Government Research Institute of Public Health and Environment Kangbuk Agro-Fishery Products Inspection cente) ;
  • Kim, Myung-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment Kangbuk Agro-Fishery Products Inspection center)
  • 발행 : 2003.10.01
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초록

This study was carried out to investigate the quality control of domestic and imported Puerariae Radix. It was analyzed by HPLC using ${\mu}$-Bondapak C$\sub$18/ column with 35% MeOH containing 1% CH$_3$3CO$_2$H system as the mobile phase at UV 254nm. Good linearity showed over the range of 10 to 200$\mu\textrm{g}$/$m\ell$(r$^2$=1) for Puerarin, and 0.5 to 10$\mu\textrm{g}$/mg(r$^2$=0.9999) for Daidzein. The average contents of Puerarin and Daidzein were 5.5${\pm}$1.2%(Domestic), 5.3${\pm}$0.7%(Imported), and 0.05${\pm}$0.02%(Domestic), 0.08${\pm}$0.02%(Imported).The average recovery rates of Puerarin and Daidzein were 101.8${\pm}$1.9% and 97.2${\pm}$0.7%, respectively.

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