Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2003.10b
- /
- Pages.169.1-169.1
- /
- 2003
Novel Cell-based Protease Assay System for Molecular Cell Biology and Drug Discovery
- Hwang, Hyun-Jin (Ahram Biosystems Inc.) ;
-
Kim, Jeong-Hee
(Kyung Hee University) ;
- Park, Joon-Woo (POSTECH) ;
- Kim, Sung-Hee (POSTECH) ;
- Lee, Min-Jeon (POSTECH) ;
- Jeong, Han-Seung (POSTECH) ;
- Hwang, In-Hwan (POSTECH)
- Published : 2003.10.01
Abstract
Recently development of cell-based assay systems which are useful in molecular cell biology and drug discovery attracts significant attention. Here, we introduce a new technologies for monitoring enzyme activity and its inhibition inside living cells. Among various enzymes, proteases are important targets for studying various biological and disease-related processes such as viral infections, apoptosis and Alzheimer's disease. In this study, a sensitive cell-based protease detection system that enables direct fluorescence detection of a target protease and its inhibition inside living cells is introduced. (omitted)
Keywords