Characteristics of Chitosanase from Aspergillus fumigatus KB-1

Two chitosanses produced by Aspergillus fumigatus KB-1 were purified by ion exchange and size exclusion chromatographies. Molecular weights of chitosanses were 111.23 KDa (chitosanase I) and 23.38 KDa (chitosanase II). The N-terminal amino acid sequence of chitosanase II was determined: YNLPNNLKQIYDKHKGKXSXVLAK(\ulcorner)GFTN. The optimum pH of the chitosanase I and II were 6.5 and 5.5 respectively. The optimum temperatures were $60^\times$C and $70^\times$. Two chitosanases were most stable at $10^\times$C. (omitted)