대한약학회:학술대회논문집 (Proceedings of the PSK Conference)
- 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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- Pages.160.1-160.1
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- 2003
Expression and Characterization of ATP-binding-cassette(ABC) Transporter in Cephabacin Biosynthesis Gene Cluster of Lysobacter lactamgenus
- Park, Myoung-Jin (College of Pharmacy, Yeungnam University) ;
- Lim, Mi-Ok (College of Pharmacy, Yeungnam University) ;
- Nam, Doo-Hyun (College of Pharmacy, Yeungnam University)
- 발행 : 2003.10.01
초록
In order to confirm the biological function of ORF10 in cephabacin biosynthesis gene cluster of Lysobacter lactamgenus as an ATP-binding-cassette (ABC) transporter, the gene for ORF10 was amplified and subcloned into pET-28a(+) expression vector. After gene induction with 0.5 mM IPTG at 30~! and further cultivation at
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