Proceedings of the Korean Society of Sericultural Science Conference (한국잠사학회:학술대회논문집)

Korean Society of Sericultural Science (한국잠사학회)
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Construction of Transfer Vector for Production of Baculovirus Occlusion Bodies that Contain Novel Recombinant Crystal Protein

  • Shim, Hee-Jin (School of Agricultural Biotechnology, Seoul National University) ;
  • Choi, Jae-Young (School of Agricultural Biotechnology, Seoul National University) ;
  • Roh, Jong-Yul (School of Agricultural Biotechnology, Seoul National University) ;
  • Li, Ming Shun (School of Agricultural Biotechnology, Seoul National University) ;
  • Je, Yeon Ho (School of Agricultural Biotechnology, Seoul National University)
  • Published : 2003.10.01
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Abstract

Baculovirus occlusion bodies have been recently engineered to incorporate foreign protein such as the Bacillus thuringiensis (Bt) CrylAc protein for improvement of insecticidal activity. In this study, polyhedrin, cylAc, egfp and crylCa genes were fused to produce occlusion bodies that contain novel recombinant crystal protein by homologous recombination between cylAc and crylCa genes in insect cells. (omitted)

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