Proceedings of the Korean Society of Sericultural Science Conference (한국잠사학회:학술대회논문집)

Korean Society of Sericultural Science (한국잠사학회)
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Mining Single Nucleotide Polymorphisms from Silkworm EST Data

  • Qingyou, Xia (The Key Sericultural laboratory of Agricultural Ministry, Southwestern Agricultural University) ;
  • Tingcai, Cheng (The Key Sericultural laboratory of Agricultural Ministry, Southwestern Agricultural University) ;
  • Jifeng, Qian (The Key Sericultural laboratory of Agricultural Ministry, Southwestern Agricultural University) ;
  • Zheyang, Zhou (The Key Sericultural laboratory of Agricultural Ministry, Southwestern Agricultural University) ;
  • Zhonghuai, Xiang (The Key Sericultural laboratory of Agricultural Ministry, Southwestern Agricultural University)
  • Published : 2003.10.01
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Abstract

We made use of 81, 635 expressed sequence tags (ESTs) derived from 12 different cDNA libraries of Bombyx mori to identify high-quality candidate single nucleotide polymorphisms (SNPs). By PHRAP assembling, we obtained 12, 980 contigs containing 11, 531 contigs assembled by more than one reads. From 117 contig sequences, which were assembled by 1, 576 high-quality reads base-called with PHRED, we identified 101 candidate SNPs and 27 single base insertions/deletions based on a neighborhood quality standard(NQS) of SNP. (omitted)

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