Proceedings of the Korean Society of Sericultural Science Conference (한국잠사학회:학술대회논문집)
- 2003.04a
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- Pages.76-76
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- 2003
Purification and cDNA Cloning of Insect Defensin from Lepidopteran Lavae, Galleria mellonella
- Jeong, Woo-Hyuk (Department of Life Science, Hoseo University) ;
- Yun, Eun-Kyung (Department of Life Science, Hoseo University) ;
- Lee, Young-Shin (Department of Life Science, Hoseo University) ;
- Kim, Iksoo (Department of Sericulture and Entomology, National Institute of Agriculture Science and Technolog) ;
- Ryu, Kang-Sun (Department of Sericulture and Entomology, National Institute of Agriculture Science and Technolog) ;
- Lee, In-Hee (Department of Life Science, Hoseo University)
- Published : 2003.04.01
Abstract
Here we report an antifungal peptide isolation from G. mellonella larvae. The peptide shows a high degree of sequence homology to an insect defensin, named heliomicin, first reported in Lepidoptera. The peptide was purified by a three-step procedure consisting of acid extraction, gel permeation chromatography and reversed-phase HPLC. First the N-terminal amino acid sequence of the purified peptide was determined by automated Edman degradation. (omitted)
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