Proceedings of the Korean Society of Sericultural Science Conference (한국잠사학회:학술대회논문집)

Korean Society of Sericultural Science (한국잠사학회)
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Purification and cDNA Cloning of a Cecropin-like Antibacterial Peptide from the Hemolymph of Wax Moth, Galleria mellonella

  • Jeong, Woo-Hyuk (Department of Life Science, Hoseo University) ;
  • Kim, Chong-Han (Department of Life Science, Hoseo University) ;
  • Lee, Joon-Ha (Department of Life Science, Hoseo University) ;
  • Lee, Young-Shin (Department of Life Science, Hoseo University) ;
  • Kim, Iksoo (Department of Sericulture and Entomology, National Institute of Agriculture Science and Technolog) ;
  • Ryu, Kang-Sun (Department of Sericulture and Entomology, National Institute of Agriculture Science and Technolog) ;
  • Lee, In-Hee (Department of Life Science, Hoseo University)
  • Published : 2003.04.01
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Abstract

We have purified and characterized cecropin A-like antibacterial peptide from the hemolymph of immunized Galleria mellonella larvae. Acid extraction, gel filtration, preparative acid urea PAGE, and reversed-phase HPLC were used for purification of peptide. The molecular mass of the purified peptide was estimated to be 4160.68 Da by MALDI-TOF mass spectrometry. (omitted)

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