Suspension culture of Stably Transformed Drosophila melanogaster S2 Cells expressing EGFP and EPO

  • Sohn, Bong-Hee (Department of Sericulture & Entomology, NIAST, RDA) ;
  • Lee, Jong-Min (Department of Genetic Engineering, Kyung Hee University) ;
  • Kim, Yong-Soon (Department of Sericulture & Entomology, NIAST, RD) ;
  • Kang, Pil-Don (Department of Sericulture & Entomology, NIAST, RD) ;
  • Lee, Sang-Uk (Department of Sericulture & Entomology, NIAST, RD) ;
  • Chung, In-Sik (Department of Genetic Engineering, Kyung Hee University)
  • Published : 2003.04.01

Abstract

Recombinant plasmids harboring heterologous genes coding enhanced green fluorescent protein (EGFP) and erythropoietin (EPO) were transfected and expressed in Drosophila melanogaster S2 cells. Stably transformed cell populations expressing EGFP or monkey EPO were isolated after 4 weeks of selection with hygromycin B. (omitted)

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