한국자원식물학회:학술대회논문집 (Proceedings of the Plant Resources Society of Korea Conference)
- 한국자원식물학회 2003년도 제10차 국제학술회의 및 추계정기 학술발표회
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- Pages.20-20
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- 2003
Isolation and culture of protoplasts from leaf tissue of Capsicum annnum var. accumnatum Fingerh and C. frutescens L. [Syn. C. minmum Roxb.] (Bird chilli)
- Lee, Kue-Jae (Faculty of Bioresources Sciences, Chonbuk National University) ;
- Lee, Wang-Hyu (Faculty of Bioresources Sciences, Chonbuk National University)
- 발행 : 2003.10.01
초록
Isolation and culture of leaf protoplasts from two chilli cultivars (Capsicum annuum var. accumnatum and Bird chilli) were developed to enhance selection process in the somatic hybridization programmes. In order to isolate the protoplasts from leaves of these two chilli cultivars different incubation periods (3, 5 and 10 hours) were tested with combinations of enzyme mixtures containing cellulase and macerozyme. Leaves were incubated on three enzyme mixtures (2% cellulase +0.4% macerozyme, 1% cellulase +0.2% macerozyme and 0.5% cellulase +0.1% macerozyme in 13% mannitol) at 251oC in the dark. Three hours of incubation using 2% cellulase and 0.4% macerozyme was the best for the protoplast isolation of both chilli cultivars tested. The yield was 5
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