한국식물병리학회:학술대회논문집 (Proceedings of the Korean Society of Plant Pathology Conference)
- 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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- Pages.148.1-148
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- 2003
First report of Cycas necrotic stunt virus from cultivated Daphne plants
- Lee, B.Y. (Plant Virus GenBank, PVGABC, College of Natural Science, Seoul Women′s University) ;
- K.H. Ryu (Plant Virus GenBank, PVGABC, College of Natural Science, Seoul Women′s University)
- 발행 : 2003.10.01
초록
Natural virus infection of cultivated Daphe odora plants showing chlorosis and stunting was observed and their causal agent was investigated. An isolate of isometic virus was purified from infected leaf tissues, and it could infect systemic severe mosaic on Chenopodium quinoa and C. amaranticolor. cDNA library was generated from partially purified viral RNAs and oligo dT primer-pSPORTl system, and recombinant clones were selected and their inserts were sequenced randomly. Nucleotide sequences of the virus were analyzed by BLAST, and it was closely related to members of subgroup B in the genus Nepovirus. The sequence analysis suggest that the virus was identified as an isolate of Cycas necrotic stunt virus (CNSV) because it was 89.7 % and 94.7 % identical to known CNSV for the CP and 3' noncoding region, respecitively. RT-PCR was performed to screen disease incidence of CNSV in Daphe plants, and five out of 10 plants (50 %) were infected by CNSV This is the first sequence information of CNSV from Daphe plants.
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