Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)

The Korean Society of Plant Pathology (한국식물병리학회)
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Complete sequence of genome RNA of Pepper mottle virus Korean isolate

  • H.I. Yoon (Plant Virus GenBank, PVGABC, College of Natural Science, Seoul Women′s University) ;
  • J, Y. Yoon (Plant Virus GenBank, PVGABC, College of Natural Science, Seoul Women′s University) ;
  • Park, G.S. (Department of Horticultural Environment, National Horticultural Research Institute, RDA) ;
  • Park, J.K. (Division of Biological Environment, Kangwon National University) ;
  • K.H. Ryu (Plant Virus GenBank, PVGABC, College of Natural Science, Seoul Women′s University)
  • Published : 2003.10.01
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Abstract

Complete nucleotide sequence of genome RNA of a Korean isolate of Pepper mottle virus (PepMoV-Vb) from field-collected diseased paprika (Capsicum annuum var grossum) was determined in this study. Symptoms of isolates of PepMoV were divided largely into two groups, vein banding (Vb) and vein clearing (Vc) patterns. PepMoV-Vb RNA consists of 9,640 nucleotides excluding the poly(A) tail. A single open reading frame was identified beginning at nucleotide position 169 encoding a polyprotein of 3024 amino acids which is typical of the Potyvirus genus. The complete nucleotide sequence and coding regions of PepMoV-Vb were compared to that of 11 potyviruses within the genus Potyvirus. The overall nucleotide sequence identity was 94.7 and 94.1％ identical to PepMoV-C and PepMoV-FL, respectively. Full-length cDNAs of PepMoV-Vbl were synthesized from purified viral RNAs by RT-PCR and their genome structure was analysed by RFLP analysis. This is the first report on complete nucleotide sequence of PepMoV isolated from paprika in Korea.

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